Distance to ocean provides a summary measure of the coastal environment of the farm

The cannabis industry has historically resisted widespread farm consolidation, perhaps due to its status as an unregulated, and illicit or semi-licit, activity. While the amount of cannabis produced in California is substantial , evidence from 2016 suggests that most outdoor cannabis was then produced on farms smaller than one acre . When Proposition 64 legalized non-medicinal cannabis in 2016, its size provisions explicitly acknowledged the state’s desire to see cannabis farms remain small . Initial regulations limited each permit to an area no greater than one acre and limited each entity to only one permit. Federal laws against cannabis have also encouraged small farms: Farmers with more than 99 plants potentially face federal minimum sentences of five years in prison . Local permitting may also favor smaller producers. Each jurisdiction in California can create its own permitting system, and possessing a local permit is a condition for obtaining a state permit. Most local jurisdictions place limitations on field sizes, and these limitations can encourage small-scale farming. While local permits may provide an avenue for local governments to protect small farmers , they also add another layer of regulation, potentially increasing entry costs. Beginning with California’s first attempt to implement a comprehensive regulatory system for the cultivation and distribution of legal cannabis, through the 2015 passage of the Medical Marijuana Regulation and Safety Act, stakeholders have expressed concerns that the permitting process privileges large farms over small. MacEwan et al. calculate that, due to the nature of regulatory costs, the type of small cannabis farmer prevalent in Northern California is the “least likely to participate in the regulated market.” Yet to date, empirical evidence on cannabis producers’ engagement with the formal market under the new regulatory framework has been lacking. In particular,hydroponic drain table there is a large evidence gap about the types of farms that participate in the regulated market and those that do not.

The gap exists partly because of a lack of public data about growers who have not applied for permits. We remedy that gap by combining information about farmers who have started the permit application process with a unique dataset of cannabis farms in Humboldt County in 2012 and 2016. Humboldt County is one of the largest cannabis producing regions in California and perhaps the world. Cannabis farming began there in the early 1960s, with rapid expansion following in the 1970s, and cannabis has been among the most valuable crops in the county at least since a proposition legalizing medical cannabis was approved by voters in 1996 . Recent studies suggest that at least 5,000 cannabis farms operate in Humboldt County . In the lead-up to the enactment of regulated cultivation of cannabis — which began for the medicinal market in 2016 and for the adult-use market in 2018 — the region experienced a cannabis boom, with the number of plants under cultivation increasing by 150% between 2012 and 2016 . This time of massive cannabis expansion is often referred to locally as the “green rush.” To track both permitted and unpermitted cannabis growers, we used data created by Butsic et al. . In their study, Butsic et al. hand-digitized cannabis farms using very high resolution satellite imagery. Cannabis production was measured in both 2012 and in 2016. Outdoor plants were counted and the number of plants inside greenhouses was estimated based on greenhouse size. Of the 1,724 farms in the dataset, 942 started producing cannabis between 2012 and 2016 and 782 produced at least some positive amount in both 2012 and 2016 . For permit data, we used publicly available data from the Humboldt County Planning Department, compiled from applications for commercial cannabis cultivation permits . We were able to combine the farm location data with the permit data based on the unique parcel identification that existed in both datasets. In total, applications were received for cultivation on 1,945 unique parcels. Of these, 533 were located within our study area .

We also include data describing farm/parcel characteristics. Locational variables such as distance to public roads and cities are used to proxy for transportation cost, while distances to endangered and threatened fish species habitat proxy for the environmental sensitivity of a site. Biophysical characteristics such as slope and presence of prime agricultural soils are used to describe the growing conditions of a site, while zoning designations are used to identify areas where growing cannabis is allowed . We also determined if a timber harvest plan had been associated with a parcel at any point since 1997. We include the quadratic term on farm size to increase the goodness of fit in our model and allow a more flexible relationship between farm size and permit application. The other covariates included in our regression are useful predictors of permit application, as they explain site-specific characteristics as well as proxy for potential land-use opportunities. They have been found to be significant predictors of farm location or farm abandonment . Importantly, these other covariates are primarily time-invariant or predetermined at the time growers decide whether to apply for permits. Specifically, we include variables of environmental sensitivity as proxies for potential challenges in obtaining approval from the Regional Water Quality Control Board. We include zoning information to help describe the other potential uses of the parcel if it were not being used for cannabis. Finally, we include a variable indicating if the area had ever had a timber harvest plan since 1997. We include this variable to see if past land use influences the likelihood of permit application. The average farm size in 2016 was 432 plants, with a median of 263 plants, a minimum of 14 and a maximum of 12,901 . Over 90% of farms produced fewer than 1,000 plants and fewer than 2% produced more than 2,000. Examining permit application rates by farm size reveals a distinct size gradient , as application rates increase substantially over farm-size categories. This pattern holds for both existing and new farms, but the rise is much sharper for the latter. Approximately 10% of small new farms apply for a permit, but rates jump to 61% and 50%, respectively, for the largest farm size groupings. We found a significant difference in size between farms that applied for a cannabis permit in 2016 relative to those that did not apply . The trend according to which larger farms applied for permits at higher rates held true regardless of production type .

The size differences are proportionally similar for both greenhouse and outdoor plants, so we do not find evidence that the relationship between farm size and permit application is solely driven by production method. Our regression models confirm that this result is robust to controlling for other covariates. In all our regression specifications, the coefficient on the total number of plants in 2016 is positive and statistically significant at the 1% level. The effect size of the number of plants indicates that, controlling for parcel characteristics, an increase of 100 plants increases the probability of applying for a permit by 2.4% , with the slope of the relationship declining for extremely large farms . The overall marginal effect is similar for existing and new farms, , though the declining marginal effect for very large farms is driven by new farms , and is robust to the inclusion of watershed fixed effects . The pattern also holds for size in 2012. Restricting the sample to existing farms, an increase of 100 plants in 2012 increases the probability of application by 3.1%.We first categorize growth of existing farms according to the proportionate change in plants produced between 2012 and 2016. The “declining production” group consists of farms that shrank by more than 5% ; “minimal change” farms experienced between −5% and 5% growth ; “moderate growth” farms grew between 5% and 50% and “high growth” farms grew by more than 50% . Within the sample of existing farms, there is a clear gradient of application rates with respect to growth between 2012 and 2016 . The farms least likely to apply are those that declined in size, followed by those with minimal growth. Application rates for existing farms that grew moderately jump to over 40%,rolling benches hydroponics with high growth farms the most likely to apply. Note that across all expansion rates for existing farms, application rates are significantly higher than the average rate for new farms. Statistical tests confirm this trend. Existing farms that applied for permits displayed a mean expansion of 212 plants between 2012 and 2016, while the mean expansion for farms that did not apply was 130 plants . This difference of 82 plants is significant at the 1% level. Our regression results also find expansion associated with permit application . In column 6, an increase of 100 plants among existing growers is associated with a 1.5% higher probability of applying for a permit, with the result positive and statistically significant at the 1% level. farms than existing farms . However, new farms are far less likely to apply for permits than existing farms. The univariate comparison shows that, on average, a new farm was 22% less likely to apply for a permit than a farm that already existed in 2012. Our regression results indicate that this relationship is robust to controlling for associated covariates, including farm size. The coefficient on new farms is statistically significant and negative in all regression specifications.

Controlling for other factors, new farms are approximately 7.3% less likely than existing farms to apply for a permit, with the magnitude of the effect slightly reduced when relying only on within-watershed variation . Small new farms are very unlikely to apply for a permit, even in comparison with existing farms of similar size . Regression results indicate that farms which have not applied for permits tend to be located further north, closer to both cities and the coast and further away from roads . They are also more likely to be located on prime agricultural soils, which is a listed requirement for obtaining a permit. However, there seems to be no effect associated with flat terrain or agricultural zones, which are also requirements for permits. These results suggest that siting criteria in the permit ordinance do not appear to be positive independent drivers of application decisions. In contrast, farms that did apply for permits tend to be located closer to streams and chinook salmon habitat, even as permit eligibility requires the use of non-diversionary water sources . Applying farms are also more likely to be located in forest recreation or timber production zones and to have been transacted at least once since 2015. They also tend be located on larger parcels. However, from comparing the results in columns and , it is clear that a number of regression outcomes between permit applications and parcel characteristics are not robust to the inclusion of watershed fixed effects. This suggests the existence of underlying geographic drivers which might influence these relationships.Cannabis has been profitably produced in California, primarily on small farms, for decades . As cannabis becomes increasingly legal, production practices have become more standardized, and many small farms fear that the increased regulatory costs associated with formalization will force them to either shut down or remain on the black market . Here, we use empirical data on farm location and permit status to investigate differences between cannabis farms that applied for permits to produce in the legal market and those that did not. We find strong evidence that farms with more plants are more likely to apply for permits than farms that grow fewer plants. This is consistent with the argument that increased formalization disfavors small-scale farms . A potential implication of this trend is that continued cannabis expansion in California may disproportionately favor the establishment of large farms, despite measures seemingly designed to prevent this outcome. Small cannabis farms may face challenges similar to those faced by small farms producing other crops — and if small farms are valued, additional policy solutions are required. While our results point toward a robust positive relationship between size and permit application , we cannot definitively attribute the cause to either the fixed cost of initial application or ongoing costs associated with regulatory compliance.

Our inability to incorporate cannabis home delivery is an important limitation of this study

Methods for operationalizing access to cannabis delivery remain undeveloped, but cannabis delivery constitutes a growing portion of the retail market, a pattern accelerated by the COVID-19 pandemic.Given that most jurisdictions banning outlets also ban delivery businesses, the associations we have observed may be relevant to delivery businesses as well, but this should be evaluated empirically in future research. Other limitations include the potential for uncontrolled confounding. We may have also underestimated effect measure modification by controlling for confounders of the policy-outlet relationship that are also on the pathway from median income or racial–ethnic composition to outlet densities. Additionally, illegal outlets may be under counted in our data in 2020, because legal action in the previous year encouraged Weedmaps to purge listings of illegal outlets. We assessed local policies cross-sectionally in 2020 and assumed them to be time-invariant over the study period. Policies may have been adopted several months or years prior to 2020. We could not assess how within-place temporal changes in policies affected outlet densities, either immediately or lagged. We modeled the temporal relationships between outlet densities and time-varying covariates such as sociodemographics, but we could not model other temporal dynamics, including whether a recreational outlet was previously medical-only versus newly opened. Reverse causation, in which local policies are adopted in response to outlet densities, is also possible. However, the cannabis norms and political orientations that determine local policies are unlikely to change substantially and systematically over the 3-year study period. We focused on a subset of California,microgreens shelving which limits generalizability. Nonetheless, our study areas captured the majority of the California population and diverse approaches to cannabis regulation.

Although block groups are very small spatial units, it is possible that analyses at other levels of spatial aggregation could produce different results . Some mismeasurement of spatial effects is possible because block groups at the edge of the study regions lacked measurements for all neighbors, but any bias is likely to be small because this concern applies to only a small minority of study areas. Finally, we define “equity” as the absence of differential associations between policies and outlets by block group median income and racial–ethnic composition, but other measures may also be appropriate. Conclusions As with all policies, cannabis legalization likely involves balancing harms and benefits. For jurisdictions that have chosen to legalize recreational cannabis, the optimal density of outlets is unknown. If lessons from alcohol and tobacco apply to cannabis, limiting outlet densities may protect public health.Alternatively, if cannabis outlets promote substitution of alcohol, tobacco, or opioids for cannabis, and these substances are less harmful than cannabis, then health may be improved.Local control of legal cannabis has resulted in considerable variation in cannabis policies across California with important implications for health equity. This analysis suggests that bans on outlets were disproportionately adopted in jurisdictions with more White residents, higher median income, and less poverty, and this pattern has resulted in the disproportionate placement of cannabis outlets in less advantaged communities. Moreover, although local policies in jurisdictions permitting cannabis outlets have the potential to address inequitable distributions of cannabis outlets, those policies adopted to date do not appear to have achieved this. Findings from this study should be incorporated into broader assessments of the costs and benefits of recreational cannabis legalization considering short-term and long-term public health and social welfare outcomes. Alternative policy and public health approaches that protect vulnerable communities from disproportionate harms related to cannabis should be explored.

Under suppressive antiretroviral therapies , infection with Human Immunod efficiency Virus remains a challenge, both due to the maintenance of cellular reservoirs and to chronic inflammation driven by low viral replication and dys regulated immune mechanisms . In end organs such as the brain, where the majority of the HIV-1 targets and reservoirs are of myeloid origin , the remaining inflammatory environment contributes to co-morbidities , including neurological and cognitive problems , particularly if ART is not introduced sufficiently early . Substance use disorders are frequent among the HIV-infected population, further contributing to cognitive impairment . Nonetheless, the mechanisms by which addictive substances and HIV interact are multi-factorial and poorly understood. Drugs of abuse impact the brain reward system, by modifying levels and balance of neurotransmitters . The HIV target cells, macrophages and microglia, as well as CD4 T cells, express receptors to neurotransmitters, so SUDs are likely to impact mechanisms of immune and inflammatory, and anti-viral responses . Biomarkers that detect the effect of SUDs, and distinguish HIV in that context, may clarify how drugs affect HIV and inflammation. Cannabis is one of the most prevalent substances among HIVþ subjects, compared to the non-infected population , either prescribed for ameliorating symptoms associated with the virus or with ART , or used recreationally, as well as a component of poly substance use , which in itself is a risk factor for HIV infection. The effects of cannabis may drastically differ from the effects of stimulant drugs such as Methamphetamine , particularly in the context of HIV infection . Yet, similar to other drugs of abuse, cannabis may be a confounder shifting the expression of biomarkers of inflammation and cognition, masking our ability to clearly measure the impact of virus, ART or other treatments in the immune status and brain pathogenesis, or may be altogether beneficial. In terms of cognition, cannabis exposure has been linked to lower odds of impairment in people living with HIV. On the other hand, impaired verbal learning and memory, may be negatively impacted by cannabis use . Other studies report no differences, or detrimental effects in HIV-negative populations, suggesting that the observed effects of cannabis, including its benefits, may be largely domain and context-dependent. It has been reported that cannabis use improves biomarkers of inflammation in the CSF and plasma of HIVþ subjects and decreases the number of circulating inflammatory cells . We have tested the value of a large panel of transcripts associated with inflammation and neurological disorders, digitally multiplexed and detectable in peripheral blood cells from HIV-positive and HIV-negative subjects, users of cannabis or not . The differences between groups were analyzed using a systems biology approach that identified associated gene networks based on pathways and molecular interfaces, for identifying and visualizing orchestrated transcriptional patterns consistent with HIV infection, CAN exposure, and their interactions.

Trends in the behaviors of gene clusters and their predicted regulators revealed that effects of cannabis differ between HIVand HIVþ groups. Moreover, mixed statistical models have pinpointed genes that are further influenced by cannabis in the context of poly substance use. These context-dependent effects of cannabis indicate the complexity of its molecular actions and properties, and the challenges of biomarker discovery in the context of SUDs. At the same time, the results suggest that cannabis in the context of HIV infection may drive benefits by promoting a decrease of pro-inflammatory and neurotoxic transcriptional patterns, changes and changes in gene clusters associated with leukocyte transmigration and neurological disorders.The impact of HIV, cannabis and their interaction on peripheral markers of cell subset, cellular function and activation was estimated using a combination of cell surface protein detection by flow cytometry and a targeted digital multiplex transcriptomic analysis. The specimens were from males, with homogeneous age and education, and the same race distribution, as shown in Table 1. The examination of clinical data revealed that in HIVþ individuals, cannabis did not significantly affect CD4 nadir, CD4/CD8 ratio, plasma or CSF viral load. Cannabis users were significantly more likely to engage in poly substance use, or use other drugs, including alcohol, cocaine and METH. HIV status significantly increased the incidence of lifetime major depressive disorders,greenhouse tables which was not affected by cannabis use . Neuropsychological data indicated that cannabis had a marginal effect on Global T scores . By flow cytometry, we verified that the specimen freezing process did not impact subset distribution . For instance, HIVþ subjects had significantly lower percentage of CD11bþCD14þ monocytes compared to HIV- subjects, particularly the ones exhibiting the inflammatory marker CD16þ, regardless of cannabis use . The percentage of CD4þ T cells was also decreased in HIVþ specimens when compared to HIV, with no effect of cannabis . The percentage of CD8þ cells, on the other hand, was significantly increased in HIVþ non-cannabis users, but not in cannabis users, compared to respective controls .Molecular markers of neuroinflammation, activation and leukocyte transmigration were measured in the peripheral blood cells under the hypothesis that cannabis use has an effect by itself and on modulating the effects of HIV. A panel of 784 markers relevant to neurological disorders and inflammation were tested by Nanostring. Of these 381 did not produce any signal in any of the specimens and were excluded from the analysis. The expression of genes with significant signal over noise in more than arbitrarily 10% of the samples was normalized by an average of 8 housekeeping genes. Hierarchical clustering performed using average normalization method applied to digital gene expression data has revealed similarities between HIV-/CANþ, HIVþ/CAN- and HIVþ/ CANþ, but all these groups were distinct from HIV-/CAN-. Clustering also allowed to identify individual specimens that showed patterns distinct from the majority within groups . Systems biology strategies were used to identify defining expression patterns in transcriptional data, and gene clusters exhibiting orchestrated behaviors perturbed by HIV infection, by the use of cannabis, or by their interaction.

We have identified significant trends in a number of gene clusters functionally annotated to biological processes and pathways of relevance to the neuropathogenesis of HIV. Overall, the analysis indicates context-dependent effects of cannabis. The majority of the digitally multiplexed genes exhibited detectable and overlapping interactions based on pathway, as indicated in Fig. 4.In cells from HIVþ/CANþ individuals, a number of genes showed decreased expression compared to HIV-/CAN- . HIV infection in the context of cannabis, revealed by the comparison of HIVþ/CANþ and HIV-/CANþ , was characterized by stronger upregulation of genes, but also several genes with decreased expression. The effects of cannabis in the context of HIV measured by the ratio between HIVþ/CANþ and HIVþ/CAN-, were characterized by a higher number of down regulated genes, and a more modest upregulation, as suggested by overall lighter orange shades. A complete list of the genes in this network and T ratio in indicated comparisons can be found in Supplementary Materials 1. Pathway-based interactions were subdivided for identification of embedded functional annotations impacted by HIV and/or cannabis, identified by DAVID Bio-informatics Resources with a gene list input. Individual functional annotations were then assembled in GeneMania for visualization of effects. A complete list of significant pathways and functional annotations can be found in Supplementary Materials 1. The pathways selected for visualization were curated based on the expression of inflammatory genes, significance to neurological disorders in the context of HIV, viral infection, pathogenesis and networks with interventional value. For instance, a gene network functionally annotated to viral host interactions was identified , where the ratio between HIVþ/CAN- and HIV-/CAN- indicated that HIV increased a number of genes annotated to that function. The ratio between HIV-/ CANþ and HIV-/CAN- , as well as between HIVþ/CANþ subjects were compared to HIV-/CANþ , indicated that both cannabis alone and HIV in the context of cannabis use increased a large number of genes in this cluster, but several genes were also decreased in both conditions, including the Ras homolog gene family GTPase RhoA, the Proteasome 20S Subunit Beta 8 , the intracellular cholesterol transporter , the E1A Binding Protein P300 and the histone deacetylase Sirtuin 1 . The ratio between HIVþ/CANþ and HIVþ/CAN- indicated that cannabis in the context of HIV was associated with a mild increase of genes in viral host interaction function , and a decrease in the general transcription factor IIB and the ubiquitin protein ligase 3A were characteristic of this comparison. Apoptosis was also identified as a relevant functional annotation , showing differential effects of HIV and/or cannabis. HIV alone decreased Caspase 7 CASP7, but increased CASP9 and the apoptosis regulator BCL2 . The effect of cannabis, on the other hand , indicated decrease in BCL2 . Likewise, HIV in the context of cannabis had a decrease in BCL2 . On the other hand, the ratio between HIVþ/CANþ and HIVþ/CAN- indicated that cannabis decreased or had mild effects on the expression of genes associated with apoptotic functions detectable in peripheral leukocytes .

A fairly large amount of work can be found on animal models of adolescent cannabis exposure

Similar to findings by Lopez-Larson discussed above, the concept of deleterious effects related to early initiation of cannabis has been explored in the neuroimaging literature as well. According to Wilson and colleagues , individuals reporting marijuana use prior to age 17 demonstrated decreased whole brain and cortical gray matter in addition to increased percent white matter volume. Findings also included higher cerebral blood flow in males reporting early initiation of marijuana use. While findings do not necessarily support a clear and consistent pattern of changes in cortical/sub-cortical volume and thickness measurements, as emphasized by Lopez-Larson and colleagues, we can conclude that marijuana may influence the trajectories of appreciable gray matter changes in several ways. The compound may illicit premature tissue development, impose a marijuana-related effect on regressive changes , and alter ongoing myelination of fiber tracts that are impacting gray matter estimates. Functional changes likely affect the mechanics that underlie structural brain changes, and interactions between these processes cannot be ruled out.White matter tissue integrity is believed to be important for efficient cortical connectivity in the developing brain. The literature has shown linear increases in white matter over early development. As the brain becomes increasingly myelinated and fiber bundles mature from infancy to late adolescence, restriction of diffusing water molecules along the principal axis of an axon is commonly observed due to increasingly compact fibers and with more limited intracellular space. Diffusion tensor imaging commonly utilizes two indices of white matter tract coherence to reflect water diffusion in white matter, fractional anisotropy and mean diffusivity , which are thought to help to identify alterations in the health of white matter fibers. Increases in FA and decreases in MD are typically seen in healthy white matter development from young children to early adulthood. In 2006, DeLisi and colleagues published one of the earlier studies to explore the potential for deleterious effects of cannabis on developing white matter.

The authors found higher FA and lower in MD in several tracts in MJ users compared to matched controls; they conclude no evidence of pathological white matter changes despite finding differences between groups. Since this study,greenhouse growing racks findings do suggest some evidence of alterations in white matter integrity in adolescent cannabis users. While DeLisi and colleagues suggest no evidence of pathology per se, subsequent studies have since shown changes in unanticipated directions. While this may not represent a typical pathological process, group differences in either direction may still be reflective of a neural alterations. For instance, increased MD in the prefrontal fiber bundles of the corpus callosum in heavy cannabis using adults who initiated use during adolescence suggest changes in white matter development associated with cannabis use. Ashtari and colleagues found that adolescents with heavy cannabis use enrolled in residential drug treatment had reduced FA and increased MD in cortical association areas such as the temporal-parietal fiber tracts. Recently, in a small sample of adolescents approximately 18 years of age, WM alterations were found in cannabis users compared to controls. Decreased FA in cortical and sub-cortical areas was found in cannabis users compared to controls with no history of substance abuse. In our laboratory, we have found white matter alterations in our abstinent teen marijuana users compared to controls. In two studies published in 2008 and 2009, we found poorer white matter integrity in several association and projection fiber tracts in adolescent cannabis users with concomitant alcohol use. Areas showing between group differences included tracts linked to fronto-parietal circuitry. White matter integrity in several of these regions was linked to neurocognitive performance on measures of attention, working memory, and processing speed; we have also seen white matter linked to emotional functioning and prospective risk taking in our substance users.

To better understand micro-structural differences in tissue integrity among adolescent marijuana users as compared to binge drinkers, we looked at white matter differences between adolescent binge drinkers compared to binge drinkers with histories of heavy marijuana use . While between group differences persisted between marijuana users and controls, surprisingly, teens engaging in binge drinking only looked significantly worse on indices of white matter integrity in several areas as compared to marijuana users, highlighting the need for further research to disentangle the effects of marijuana and alcohol on the developing brain. In general, research points to poorer white matter integrity in adolescent marijuana users compared to non-substance using controls. While white matter findings are subtle in nature, we have observed poorer white matter integrity correlated with poorer neurocognitive functioning in our studies, which underscores the impact that slight alterations in white matter health during this time could have on optimal cognitive functioning. Interestingly, some preliminary evidence supports that marijuana-related toxicity on white matter integrity may be more modest compared to the impact adolescent alcohol use has on the developing brain, although more research in needed in this area.Changes in cognitive performance after acute and longer-term cannabis use are fairly well documented, even if residual effects are suspected to largely resolve. However, less is known on how brain functioning, or neural activation/signaling, may be changed by marijuana use and thereby reflected in declines in neuropsychological performance. Comparisons between blood oxygen dependent signal in adolescent marijuana users and controls in response to cognitive tasks have revealed subtle differences in brain activation patters in marijuana users. Jacobsen and colleagues were the first to pilot an auditory working memory fMRI study comparing marijuana users compared to a tobacco using group and control group. The authors found cannabis users performed the task less accurately and failed to deactivate the right hippocampus across conditions. In another study by the same authors, nicotine withdrawal elicited increased activation across brain regions in the marijuana group, including parietal cortex, superior temporal gyrus, posterior cingulate gyrus, and the right hippocampus. The same effect was not found in the tobacco-only control group suggesting marijuana use may lead to developmental changes masked by nicotine use.We have conducted several BOLD fMRI studies evaluating differences in activation patters between our sample of abstinent marijuana users and matched controls.

In 2007, we found marijuana users to have substantially more activation than non-using peers in response to an inhibitory processing task, particularly in parietal and dorsolateral prefrontal cortices, suggesting additional neural resources required to maintain adequate executive control during response inhibition. In evaluating response patterns to a spatial working memory task, adolescent marijuana users exhibited increased activation in the right parietal lobe along with diminished activation in the right dorsolateral prefrontal cortex to achieve good task performance, which was not observed in controls. In a follow-up investigation using the same spatial working memory task, we evaluated teens with more recent abstinence compared to prolonged abstinence from marijuana, as well as matched controls. Recent users showed greater brain activation in prefrontal cortices, regions needed for working memory processes, and bilateral insula. In response to a third task assessing verbal encoding, marijuana users demonstrated increased encoding-related activation in anterior brain regions as compared to decreased activation in posterior regions, despite no differences in task performance; findings may suggest increased recruitment of neural resources in brain areas sub-serving task-related processing in marijuana using teens. Several recent studies outside of our laboratory have shown similar findings. For example, Jager and colleagues evaluated boys with frequent cannabis use compared to matched controls and found that cannabis users showed excessive activity in prefrontal regions in response to a working memory task, studies from this same research group with young adults have yielded similar, although modest, aberrant findings of the working memory system. In 2010, an investigation comprising chronic marijuana users and matched controls , suggest increased activity in the prefrontal cortex in response to a task requiring executive aspects of attention. Cousijn and colleauges recently found increased activation in heavy cannabis users in response to the Iowa Gambling task during win evaluations in brain areas such as the insula, caudate, and temporal gyrus, which was also positively related to weekly cannabis use; win-related increase in brain activity also predicted increased cannabis use six months later.Lopez-Larson and colleagues found differences in cortico-cerebellar activity in older adolescents with heavy marijuana use. The authors describe decreased activation in response to a bilateral finger-tapping task,vertical hydroponic garden and motor function activation was negatively correlated with total lifetime marijuana use.

Age of onset also continues to play an important role, as early-onset cannabis users demonstrated increased activation in the left superior parietal lobe in response to a verbal working memory challenge , and earlier initiation of use was associated with increased BOLD activity. The majority of findings suggest increased recruitment of neural resources in brain areas sub-serving task-related processing in marijuana using teens. There has been limited research on brain functioning using EEG among adolescent cannabis users. The strength in using EEG is the degree of temporal resolution that is not possible with BOLD imaging. Information on the degree of attentional bias to marijuana cues may provide some indication of brain-based differences in cue-reactivity resulting in heavier use of marijuana among certain teenagers. For instance, one lab based paradigm of cue reactivity found increased skin conductivity among teens diagnosed with cannabis use disorde. Nickerson and colleagues found that among adolescents ages 14–17, P300 response was larger among cannabis users, and response increased in the user group after handling marijuana paraphernalia; findings suggest attentional bias, increased arousal, and possible neural differences that may elucidate discrepancies among teen substance use engagement. The neurovascular effect of marijuana use in adolescence has not been studied extensively. Understanding vascular changes in cerebral blood flow can help us better understand neural signaling and vascular alterations that may be related to changes in neurocognitive functioning and/or changes in neural signaling related to the BOLD signal. Adult studies typically report increased CBF after acute exposure and lower or stabilized CBF after a period of abstinence in heavy users, although this has varied to some degree. To our knowledge, there has only been one study in adolescent blood perfusion in heavy cannabis users. In a recent study in our laboratory utilizing arterial spin labeling , we found that heavy marijuana users assessed pre-and post 28 days of monitored abstinence showed reduced CBF in 4 cortical regions, including the left superior and middle temporal gyri, left insula, left and right medial frontal gyrus, and left supramarginal gyrus at baseline; users showed increased CBF in the right precuneus at baseline, as compared to controls. We did not observe group differences in neurovascular functioning after four weeks of abstinence, suggesting marijuana may influence cerebral blood flow acutely with a possible return to baseline with prolonged abstinence. A study evaluating young adults found that acute THC administration increased blood perfusion in areas important for emotional and cognitive processing, such as the anterior cingulate, frontal cortex, and insula, and reduced perfusion in posterior brain regions. Resting state activity was also altered, as THC increased baseline activity. Very few studies have looked at neurochemical brain changes related to marijuana use in adolescence. Prescott and colleagues found decreases in metabolite concentrations in the anterior cingulate, suggesting poorer underlying neuronal health in adolescent marijuana users, While the exact mechanisms by which cannabis would affect neuronal health is unclear, it is possible that modulation of neurotransmitters such as glutamate and GABAhave adverse consequences on cellular development and neuron integrity; changes in neuronal health is one suggested mechanism which may underlie neuroimaging and neurocognitive findings discussed above.A detailed analysis of the preclinical studies is beyond the scope of this review, however briefly discussing the existing literature is important for translation to human models. Studies also focus on various cannabinoids beside Δ9 -tetrahydrocannabinol , the principal psychoactive component of marijuana; for example increasing attention is being given to cannabidiol, a nonpsychoactive cannabinoid that may have promising therapeutic effects independent of THC. However, this brief summary will focus on models of exposure to the natural compound or cannabinoid agonists, which mimic the structure Δ9 -THC. A great benefit of animal studies is lack of heterogeneity that corresponds with human consumption and substance use reporting. In animals, postnatal days 28–49 correspond with human adolescent development. Studies during this postnatal time period in rats have evaluated both emotional behavior as well as cognitive/behavioral functioning.

Should cannabis production be allowed in cities and in unincorporated towns?

Understanding this dynamic is important for local governments as they develop land use policies to govern when, where and how much cannabis production is permissible . Cannabis production’s effects on neighbors is an important point for local government officials to consider as they develop and adopt new policies to encourage the transition of black-market cannabis operations into compliant operations. The effects of cannabis production on neighbors is also important to consider while formulating policies to mitigate unintended consequences — such as unwanted odors and nighttime lights — which can exacerbate land use and social conflicts. For example, should cannabis be allowed on lands zoned for timber production or prime agriculture? What areas are compatible or incompatible with cannabis? Increased cannabis production can directly or indirectly affect traditional agriculture and timber producers. Over the last decade, cannabis cultivation has expanded rapidly in rural communities, with many cannabis farmers having moved only recently to the areas where they grow . These new arrivals are sometimes described as green rush growers. Conflicts can arise if new growers, who are often unaware of community norms, don’t manage workers appropriately, control dogs, close gates, help maintain shared roads — or if, in other ways, they complicate operations for traditional agricultural producers. Likewise, even cannabis producers who have been in business for many years — including some whose families have grown cannabis for two generations — may hold different views of rural life than do traditional agriculture and timber producers . In addition, while cannabis is now legal in California, many cannabis farmers still grow outside the regulated system, and some traditional agricultural producers may retain the sense that illegal activity is negatively affecting their community.

In recent years, the environmental impacts of hydroponic systems for cannabis cultivation have been a matter of increasing focus in California, and traditional agricultural producers and other community members have voiced concerns about water diversions , pollution from chemical fertilizers , the impacts of pesticides on wildlife , light pollution and forest fragmentation . Concerns have also arisen regarding negative impacts on local livestock producers and challenges for public land managers attempting to control trespass growing operations . At the same time, cannabis cultivation can contribute to community well-being in a variety of ways. It can bring economic gains to rural areas where the timber, livestock and fisheries industries have experienced declines. For example, cannabis cultivation can provide new business opportunities to traditional agricultural producers in the form of heavy equipment work, firewood sales, trucking, forest management or construction services. In addition, cannabis production may help buffer population declines such as those experienced in many of California’s rural areas over the last 20 years; in particular, rural schools may benefit from the enrollment of cannabis growers’ children. More broadly, cannabis farmers can bring new energy to rural communities through engagement at schools, volunteer fire departments and other points of gathering. Traditional growers’ perceptions of cannabis farmers can vary based on several factors, including the scale at which cannabis farmers operate. Scales of operation have expanded greatly over the last 20 years. Some cannabis farmers produce a few plants for personal use, others augment their incomes by growing moderate amounts of cannabis and still others grow on an industrial scale, with multiple operations on numerous parcels. All scales of operation include both regulatory-compliant growers and black-market growers. One might expect traditional agricultural producers to regard these different varieties of cannabis growers differently. But large landowners are themselves not homogenous — for example, some are absentees. In this research we hypothesized that absentee landowners would have different experiences and perceptions of the cannabis industry than do traditional producers who live on their land. Humboldt County and many communities around California are currently setting ordinances to manage legal cannabis production. But as they do so, little is known about the potential interaction of cannabis with traditional agriculture and timber producers and whether these industries are compatible. Information about the effects of cannabis production on traditional agricultural producers may be helpful to policy makers because traditional producers are often important contributors to rural economies and stewards of public trust resources such as wildlife and clean water. We conducted this research with the goal of determining how larger landowners — who, in Humboldt County, are generally timber or beef producers — experience and perceive cannabis production.

We surveyed by mail all landowners in Humboldt County who own at least 500 acres . We asked a series of questions about landowner experiences with the cannabis industry and how the industry directly affected landowners’ economic well-being, community, property and personal safety. We also asked how, in their view, the cannabis industry influences the community and the environment. We asked landowners to provide their views on grower demographics and on changes in their communities over time. In addition, we compared the experiences and perceptions of absentee and nonabsentee landowners.Humboldt County has long been among the leading cannabis-producing regions in the United States . Located on the North Coast of California, Humboldt County is characterized by steep terrain and a Mediterranean climate; a climatic gradient runs from the cooler and wetter coastline to the drier and warmer inlands . Humboldt County’s agricultural and timber industries are significant in scale, with agricultural production amounting to $326 million in 2016 and timber production amounting to $70 million in the same year — although the timber numbers are down from a decade ago. These agricultural production numbers do not include cannabis production revenues, but recent estimates put cannabis production in the larger Humboldt, Trinity and Mendocino region, known as the “Emerald Triangle,” at $5 billion annually . Humboldt County is home to numerous species of concern — including threatened and endangered salmonids, spotted owls, marbled murrelets, fishers and so on — that are protected under the U.S. Endangered Species Act . Cannabis cultivation occurs within these species’ habitat areas, including in locations near and adjacent to old-growth redwood and Douglas fir forests. The intent of the survey was to understand how cannabis production in Humboldt County was affecting traditional agricultural producers, and therefore we focused only on landowners with enough property to derive a large percentage of their income from agriculture and timber activities. We identified landowners with at least 500 acres by combining land use and tax roll data. In total, 211 landowners fit this description. Landowners were mailed a paper survey, along with a stamped, pre-addressed envelope in which to return it, in January 2018. After 3 weeks, follow-up postcards were sent to landowners who had not returned their surveys. In total, 71 landowners responded to the survey . Of these, two landowners reported owning less than 500 acres and one landowner did not confirm meeting this minimum standard; we did not include these three surveys in our analysis. All survey responses were anonymous. Surveys were organized into three sections. One portion of the survey asked landowners about their direct experiences with the cannabis industry, asking them to agree or disagree with 22 statements that corresponded to four themes: how the cannabis industry has affected the economics of their operations ; how cannabis has impacted their local community ; how cannabis has affected their properties and how cannabis has affected their safety .

The surveys asked landowners to respond to each statement using a five-point Likert scale, with responses ranging from strong disagreement to strong agreement . Respondents could also respond “NA” to statements that did not apply to them. Additionally, respondents were given space at the end of each subsection to provide comments or examples. In another section of the survey, we tested respondents’ perceptions of cannabis by asking them how they felt about certain cannabis-related issues and whether cannabis cultivation has had positive or negative impacts on their communities, specifying that their responses should not necessarily be based on their personal experiences. We provided 36 statements that corresponded to four themes: community ; the environment ; changes over time in property values, community safety, community demographics and so on and grower demographics . Respondents were asked to agree or disagree with the statements using a 5-point Likert scale and were able to provide comments after each subsection. The third section of the survey solicited background information about each respondent. Respondents were asked whether they earned income from timber, ranching or dairying, how long their families had owned the land they worked and whether they were absentees. In addition, we asked landowners if they had been approached about selling their land for cannabis cultivation and if they had next-generation succession plans for the family ranch or timber business. We also asked if landowners knew of nearby indoor hydroponics cannabis growing. As indicated previously, all respondents included in our survey owned at least 500 acres of land. Twenty two percent owned between 500 and 1,000 acres, 51% owned between 1,000 and 5,000 acres and 28% owned more than 5,000 acres. Of the 69 landowners whose responses were included in our results, 63 respondents managed timberland and 56 respondents managed ranch land, meaning that most respondents managed both land types; only one respondent was involved in dairy farming. Forty-six percent of respondents lived on their properties full time, while 20% lived on their properties part time. Thirty-three percent of respondents were absentee landowners. In general, the land represented in the survey had been in respondents’ families for a long time — more than 50 years in 81% of the cases, 25 to 50 years in another 10% of the cases, less than 25 years in 6% and less than 5 years in only 3% of the cases. Fifty percent of respondents reported that their primary income was from traditional forms of agriculture or timber production; no respondents reported cannabis as their primary income source.Seventy-one percent of landowners reported that they did not grow cannabis on their property while 18% reported that they did. These percentages, however, are derived only from the 34 of 69 respondents who agreed or disagreed with the statement that they had used their property to grow cannabis. The remaining respondents — half the total — chose not to indicate whether they had grown cannabis, potentially indicating landowners’ reluctance to associate themselves with the cannabis industry.

About 40% of respondents had indirectly profited from cannabis through off-farm work such as heavy equipment work, trucking and so on . Fifty-seven percent of all respondents agreed or strongly agreed with the statement that “the cannabis industry has negatively affected my livestock operations,” while 27% disagreed with this statement. Over 60% of respondents agreed that cannabis had increased the cost of labor. Comments that respondents offered on the cost of labor included “Property values are inflated by the cannabis industry, hence costing us more for leases and ownership.” Seventy-five percent of respondents agreed or strongly agreed with the statement that “shared roads have been degraded by cannabis growers” and 65% agreed that noise pollution has increased due to cannabis growing. Fifty-five percent of respondents agreed that growers increase light pollution and 71% reported having experienced illegal garbage dumping by cannabis growers on or near their property. Forty percent of landowners disagreed or strongly disagreed with the statement that “I know growers who have values that align with my own” . At the same time, 34% of respondents agreed or strongly agreed with that statement . One respondent added that “[M]onetary impact is obvious. Cultural and moral impacts are terrible.”Fifty-six percent of respondents agreed or strongly agreed that water sources have been impacted by cannabis growers, while 25% disagreed with this statement. Fifty-six percent also agreed that water had been stolen from their property. Seventy-two percent of respondents had experienced trespassing, while 20% had not. Forty percent of respondents reported that their fencing or infrastructure had been destroyed by cannabis growers, though a similar percentage had not. Fifty percent of landowners reported that neighboring growers had failed to assist with fence maintenance, and 75% of landowners reported having discovered trespass grows on their property . One respondent added that “[Growers’] dogs killed our cattle. My brother confronted a grower in fatigues carrying an assault rifle on our property. [Our] fences have been wrecked, roads damaged, and stream water theft.” Another respondent wrote that “Yes, this is true in the past, but with the pot market collapsing I don’t think this will be a problem in the future”.

The general population may support the legalization of recreational cannabis for a variety of reasons

There are many rare social facts, but some become pathological based due to their infrequency, and others do not. Why is that so? To tailor the Durkheimian theory and make it more suitable for my empirical analysis, I incorporate Mary Douglas’s idea of pollution and power. Rather than speaking about pathological forms, Douglas focuses on marginal conditions. Her basic premise is that objects, practices, behaviors, and ideas that do not fit the existing social classifications are considered polluting, impure, and even dangerous and thus should be separated . Managing spatiality is a technique of power that allows the legitimate authority to reject “inappropriate” elements and protect what it deems normal, natural, and right. In this paradigm, space is not value-free but constructed through politics and power relations. Take, for example, racial segregation, building the wall to isolate immigrants, hot spot policing, skid rows, mental hospitals, jails and prisons—in all these cases, devaluation of individuals involves their spatial separation. Socio-spatial stigmatization is a mutually constitutive process, in which places inherit the stigma of persons, but persons also can be stigmatized through their interaction with places . For instance, concentrating homeless shelters into specific areas of a town tends to reinforce the stigmatized understanding of such areas. In similar ways, the stigma attached to a homeless shelter extends to individuals using it.Those who live in areas with a high concentration of “disordered” facilities, practices, and individuals tend to oppose them physically, ideologically, and discursively . For example, in his research of addiction treatment clinics in Toronto, Christopher Smith shows that residents perceive these facilities as a threat to the productive places and try to enforce certain socio-spatial borders .

Previous research showed that medical cannabis drying trays dispensaries were more likely to be located in less desirable parts of a neighborhood, characterized by high poverty level, unemployment, and homelessness . However, we know very little about the recreational cannabis facilities: Are they perceived as a “matter out of place”? Do they blur, contradict, and otherwise confuse the moral and social order of the communities? This study investigates the extent to which cannabis is normalized in California. Normalization is a barometer of changes in social behavior and cultural perspectives . Drawing from the Thomas theorem—stating that if men define situations as real, they are real in their consequences—I suggest that if cannabis is conceived as legitimate, it will not be pushed to the geographical and/or social margins. By the same token, if it is viewed as dangerous and illegitimate, then cannabis dispensaries will be regarded as sites of contagion, which are to be marginalized and isolated. I conduct a regression analysis to identify factors that explain variations in cannabis practices at the city level. In particular, I examine the relationship between the support of cannabis legalization in California cities and the number of cannabis licenses issued by local governments. Following the normalization theory, I expect that cities whose residents supported cannabis legalization are more likely to permit legal cannabis dispensaries within their borders. If residents view cannabis as legitimate and socially acceptable, local governments will favor cannabis-related activities on their territories. I also expect homogeneity in the characteristics of cities whose citizens supported cannabis legalization and those that permitted cannabis businesses. For example, if cities whose residents voted for cannabis legalization have a higher percentage of the middle and upper class, then cities that de facto legalized cannabis would also have a higher percentage of the middle and upper class. Since cannabis businesses create jobs and bring tax revenues to city budgets, local governments have strong incentives to permit cannabis-related activities, especially when most citizens favor legalization. But imagine situations in which citizens voted for cannabis legalization, but governments forbade any cannabis businesses, or, on the contrary, citizens did not support the legalization, but governments adopted pro-cannabis policies. These examples demonstrate the dissociation between the public’s wishes and the government’s deeds and cast doubts on the legitimacy of cannabis in a given jurisdiction. As I discussed earlier, cannabis users and distributors bear a stigma that can potentially extend to other people and places.

Prosperous communities may decide to distance themselves from the possible harm of cannabis stigma and forbid any cannabis related activities . In contrast, for economically disadvantaged communities, financial benefits may outweigh the harm of stigma and reinforce the marginalization of places with already limited resources. I look at the adoption of pro-cannabis regulation as an example of morality policies, through which local governments draw a boundary between “pure” and “polluted”, “ordered” and “disordered”, “safe” and “dangerous.” To get a more nuanced picture of the legalization and normalization processes, it is important to understand the moral-economic rationale behind decision making at the city level. The question is not only whether cannabis is legal, but where, how, and to what degree it is legal. The goal of the quantitative analysis is to reveal variations in local regulation practices and define factors impeding or fostering the adoption of permissive cannabis policies at the city level. By exploring which cities are more likely to allow cannabis businesses, this research contributes to understanding the relationship between legitimacy and legality and helps determine the current status of cannabis in California. Moreover, the focus on city-level data provides an insight into how boundaries of normality vary across local contexts. Acting as moral entrepreneurs, local governments rely on principles of the politics of pollution and create a cognitive map of acceptable and non-acceptable places . Previous studies have highlighted the importance of religiosity, economic development, political competition, community composition, organizational perviousness, and historical legacies in explaining moral policy outcomes . This research takes a different path and sets out to clarify the relationship between changes in public attitudes and the adoption of morality policies . I posit that greater social and cultural accommodation of cannabis can explain permissive cannabis policies only to a certain degree. Socio-economic characteristics of communities and their racial composition also matter. Licensing agencies collect information at the individual level.For the current project, I aggregate the number of issued licenses at the city level, which excludes any personal identification from the dataset . The reasoning behind aggregating data at the city level is that according to the Medical and Adult Use Cannabis Regulation and Safety Act , cities have the full power and authority to enforce cannabis regulation and complete responsibility for any regulatory function relating to the licensees within the city limits.Local jurisdictions decide whether cannabis businesses will be legal on their territories or not, define which types of cannabis activity to allow , and establish regulatory schemes for activities involving growing or selling cannabis.Before applying for a cannabis license, an applicant has to obtain a permit from the city administration that would enable him/her to conduct commercial cannabis activity. The permit does not guarantee each applicant a cannabis license, but it gives him/her the green light to advance to the final stage and submit the application to a licensing agency.

The dependent variable has three different measures: the total number of cannabis licenses, the number of cultivation cannabis licenses , and the number of sale and distribution cannabis licenses . I suggest that factors explaining the permissiveness of local governments towards cannabis cultivation and cannabis distribution are not exactly the same. Cultivation primarily occurs in private spaces and thus is hidden from the public eye. On the contrary, retail is associated with public display: shop-windows, street signs, and advertising boards make cannabis dispensaries visible and accessible. I expect that the public display of cannabis will be more stigmatized than its private cultivation. The normalization of cannabis is a gradual process, and we cannot expect it to progress at the same pace in different localities. But we can assume that cities whose residents supported cannabis legalization will be more likely to pass pro-cannabis laws. As seen in Tab. 2, 72% of California cities supported the legalization of cannabis in 2016, but only 45% of them legalized cannabis-related economic activities within their borders. Moreover,of those cities whose residents did not support Proposition 64, 22% eventually permitted cannabis companies,what is need to grow marijuana despite the lack of public support . There is an obvious gap between people’s preferences and governments’ actions, which should be explained. Before turning to the description of other independent variables, I should address the issue of moral hypocrisy. Greater cultural acceptance of cannabis does not necessarily translate into moral acceptance of its sale and use. In particular, we do not know whether people who supported legalization are amenable to cannabis dispensaries in their neighborhoods—i.e., we cannot exclude the NIMBY phenomenon.The willingness to legalize cannabis may follow a pragmatic logic: decriminalizing cannabis generates tax revenues, creates jobs, and diminishes law enforcement costs. People may also support legalization because it gives an opportunity to begin repairing the damages caused by the criminal justice system in the past. Moreover, it may be perceived as a progressive move that fits general liberalizing trends, including same-sex marriage, abortion, pre-marital sex, drinking, gambling, and so on. And yet, people may be moral hypocrites: they may support the idea of cannabis legalization and act in discord with it by opposing the location of cannabis dispensaries in their backyards. The statistical analysis cannot account for these nuances and, thus, simplifications are inevitable.For the purposes of this analysis, “legitimacy” means tolerance of cannabis use rather than its total acceptance; it is what people are ready to declare publicly rather than act upon. Legitimacy is a necessary but not sufficient condition for legality.

What other factors can explain the responsiveness of local governments to morally controversial issues? Since only 38% of California cities allowed cannabis companies, the dependent variable has many zeros. To account for excessive zeros and overdispersion, I use a negative-binomial regression, which allows the variance to exceed the mean and is appropriate for analyses with a count outcome variable.87 Table 4 reports the estimated coefficients of the negative binomial regression models. Model 1 contains all cannabis licenses issued in California cities in 2018 and 2019. Model 2 tests hypotheses only for licenses issued by the California Department of Food and Agriculture. Model 3 tests hypotheses only for licenses issued by the Bureau of Cannabis Control . The analysis demonstrates that cities whose residents supported cannabis legalization are more likely to permit cannabis-related activities within their borders . It is not surprising since, as I mentioned above, 45% of cities supporting cannabis legalization allow legal cannabis companies. The main question is: What other city properties are associated with the adoption of pro-cannabis legislation? Opinion polls show that the middle- and upper-class representatives, young adults, and non-Hispanic citizens support cannabis legalization at higher rates than other social groups. I ran a separate model regressing the percent of support for Proposition 64 on the index of economic prosperity, percent of people aged 20 to 29, and percent of the Hispanic population . The results confirm that the support of cannabis legalization is associated with a higher index of economic prosperity, a larger percentage of young adults, and a lower percentage of the Hispanic population. This association is significant at the 0.01 level. However, as we see in Table 4, cities that eventually allowed legal cannabis companies, on the contrary, are more likely have a lower index of economic prosperity , a lower percentage of young population , and a higher percentage of the Hispanic population . The disparity between the demand and supply offers an intriguing puzzle. Economically prosperous cities, on average, express higher support of cannabis legalization, but it does not mean that they are more likely to permit legal cannabis companies within their borders. Moreover, there are significant differences between licenses issued for sale and cultivation. Cultivation licenses are more likely to be issued in cities with a lower percentage of the young population, which can be explained by the fact that these are mostly rural remote areas, and young adults live in more urbanized places. Sale licenses are associated with three other factors: a higher percentage of the Hispanic population, a lower city’s fiscal score, and higher violent crime rates. There is substantial evidence in the findings that socio-spatial stigmatization of cannabis persists despite its legalization.

This ambiguity impedes the formation and establishment of a new universal meaning of legal cannabis

To a great extent, the press and television adopted the presidential definition of drugs: although some journalists and activists were critical of the government’s solution to the drug problem, they did not question the use of the “war” metaphor and thereby reinforced the existing perspective . Businesses, public organizations, and ordinary citizens have embraced the rhetoric of US presidents and mass media, holding urban ethnic minorities responsible for the creation of the drug problem and accountable for its resolution . The result of these rhetorical battles was more generous funding of law enforcement agencies and the growth of the prison population. Law enforcement agencies’ budgets increased.Nowadays, cannabis use for recreational and medical purposes is becoming more mainstream. According to Gallup, support for legalizing cannabis grew from 12% in 1970 to 68% in 2020.34 As Simon ironically comments, “We will perhaps have arrived at the ‘tipping point’ when baby boomers are more anxious about access to medical marijuana for their chemotherapy than if their kids are lighting up after school” . Even though cannabis has been to a great extent legitimate in the eyes of large parts of the population, the dynamics of governing through crime has not changed. While state cannabis laws gradually become more permissive, federal law enforcement remains punitive. According to FBI statistics, in 2017, cannabis was still responsible for over 40% of all drug arrests. The racial consequences also remained despite the advance of legalization: African Americans are more likely to be arrested for driving under the influence of cannabis, possession of cannabis by youths, and public consumption of cannabis indoor grow system. What can we take from the “told” history of cannabis? As I show above, the sociological and sociolegal literature presents the criminalization of cannabis as a moral issue.

Many studies provide evidence that the war on cannabis was a moral crusade that undermined the medical professionals, discredited scientific research, and spread fears among the population. In this constructivist approach to the drug problem, anti-cannabis rhetoric functions as a currency in political campaigns: the purpose of the war on cannabis was not to counter actual drug crime or drug abuse but to respond to white middle-class anxiety towards immigrants, minorities of color, or political activists. By performing a symbolic—rather than instrumental—role , anti-cannabis legislation drew a line between “normal” and “pathological” substances and between deserving and undeserving citizens. Sociology of deviance defines several essential characteristics of morality policies.First, moral laws are centered around controversial questions on which it is difficult to reach a compromise. These are a product of culture wars upon which individuals project their ideals of good life . Since different groups have opposing and even mutually exclusive concepts of virtue, moral issues tend to recur now and then and rarely come to an ultimate resolution. Second, moral laws seek to establish a set of values that dominate at their present time. According to James Hunter, laws are not just about what we can and cannot do; laws “contain a moral story that proclaims the ideals and principles of the people who live by them” . Through lawmaking, societies create “a particular nomos, a normative universe that draws distinctions, discriminates, judges, excludes and includes” . Third, passing moral laws involves the work of “moral entrepreneurs,” the rule creators and rule enforcers who invest their efforts in constructing a new meaning of goodness . Moral crusaders believe that what they do is good for others and that by improving morality, they help people live a better life . Moral entrepreneurs can have differing agendas—prohibitionist, abolitionist, humanitarian, traditionalist, liberal, etc.—but their ultimate goal is to change the existing rule. Fourth, morality policies are often difficult to enforce because the policy’s objective is symbolic and offers no clear operationalization . The application of moral laws does not affect actual behavior—for example, it does not decrease drug consumption rates—but instead shies it away from public display, encouraging the development of the black market.36

We tend to think about moral politics as banning certain activities rather than allowing them. This research, on the contrary, focuses on the legalization of cannabis as an example of permissive morality regulation. The moral dilemma that characterizes the current cannabis debate can be described as “social expectations vs. core individual rights.” As the history of cannabis criminalization shows, prohibition stood as a symbol of the general system of values with which the conservative white majority was identified. The Protestant ethics measured men’s moral worthiness in terms of productivity: any activity distracting an individual from being productive is a waste of time. Using cannabis for pleasure is immoral not only because it can affect an individual’s physical well-being but also because it represents a particular lifestyle and attitude toward work and social responsibilities. In turn, the legalization rhetoric centers on the discussion over the limits of state intervention in the private behavior of citizens.The US Constitution guarantees the right to privacy and non-interference by the state in personal matters, and the California Constitution lists a right to privacy among the inalienable rights .The history of cannabis criminalization shows that ideas and moral visions have been a decisive factor in the adoption of anti-cannabis legislation. Currently, California is in a transition period when cannabis use is slowly getting normalized in public perception but continues to be illegal and pathological from the federal government’s perspective.Analyzing the context of the war on drugs is important for understanding the departure point of this ideational change. However, as I argue in the next section, in order to apprehend today’s status of cannabis, we should turn away from the macro-level explanations and shift the attention to local actors and on-the-ground processes. The crime control paradigm is preponderant in the socio-legal literature on drugs.

The social history of cannabis in the US is often recorded as a top-down, event-based analysis, which focuses on capstone legislations, elite actors, and political intentions. From this perspective, the war on drugs results from the deliberate actions of the state officials and the mass media against marginalized groups. Although these views are extremely valuable for understanding the deep historical forces of mass incarceration, the focus on top-down processes may sometimes overshadow other possible interpretations of the drug problem. By devoting much attention to the public debates over the dangers of cannabis, these studies tell us a great deal about political agendas but relatively little about the role of medical professionals, pharmacists, manufacturers, associations, businesses, schools, families, local authorities, cannabis users and distributors in defining cannabis. Drugs are not only a criminal justice issue but also a societal problem, a medical problem, a moral problem, a market problem, and so on. As Joseph Spillane points out, much of the action happens “on the street, in the daily interactions among sellers, users, families, doctors, police, and jailers” . As I argue further, the prevailing orthodoxy often fails to understand criminalization as a complex dynamic process with several levels of action and thus casts aside many essential questions. One voice that is consistently missing in the constructivist approach to the drug problem is the voice of drug addicts and their immediate environments. As Michael Fortner argues, the existing perspectives on the war of crime tend to minimize the agency of African Americans who are typically portrayed as victims of the power of racial order and reactionary Republican politics. The New Jim Crow’s thesis focuses on white power and black powerlessness, which renders black politics invisible and obscures the causes of mass incarceration . According to Fortner, many scholars treat actual crime as fiction rather than lived experience. To cover the theoretical gaps, he investigates the role of the “black silent majority” in adopting punitive legislation, namely the Rockefeller drug laws in New York City in 1973. His study provides several critical analytical implications. First, we cannot study the drug problem out of context without exploring how local institutions and local processes influenced the framing of public concerns and policy responses . Second, aggregated statistics give little information about attitudes to crime and drugs. As Fortner demonstrates, white districts in New York City experienced significantly lower violent crime rates, drug addiction, and deaths due to drugs than black areas. The devastating effect of the drug and crime problem on black communities raised great concerns among the black population and led to higher support of anti-drug and anti-crime policies.

Evidence from California reveals similar patterns: in the 1970s, whites in Los Angeles were more concerned with pollution and noise than crime, while black citizens listed better crime control as the number one issue . This picture contradicts the popular notion in socio-legal studies that whites supported punitive policies more than blacks. By focusing on white victimization and black criminalization, researchers neglected the activism of the urban black middle class, which created incentives for local politicians to respond to demands for greater punitive policies .James Forman advances a similar argument showing that working- and middle-class black communities did not support the decriminalization of cannabis. The history of cannabis is often presented as a part of the war on drugs. However, as Forman righty notices, the anti-drug campaign aimed at heavier drugs. Nixon’s declaration of drugs as the nation’s largest enemy coincided with the first attempts to decriminalize cannabis at the state level by making possession of small amounts of cannabis a civil fraction rather than a criminal offense. The widespread knowledge about the minimal risks of cannabis use boosted decriminalization movements in many states. Since the pro-cannabis movements’ leaders were overwhelmingly white, cannabis equipment decriminalization was framed as a question of civil liberties and individual autonomy rather than racial justice. In Washington, D.C., the black community took cannabis decriminalization with skepticism as a policy that protects young whites and oppresses young blacks. White teenagers could smoke cannabis without jeopardizing their future because their middle-class cocoon could shield them from the consequences of drug use. But, poor black teenagers had much less room for error because they would risk graduating from school or finding a job . In other words, to the African American communities, the drug problem was not just a hypothetical threat. The 1960s the heroin epidemics had instilled a real sensibility for the drug problem and taught the members of black communities that addiction could destroy families, schools, and entire neighborhoods. Another particularity of the crime control perspective is the reliance on law and legislation as a sole historical marker of change . The “told” history of cannabis prohibition is often plotted as a sequence of events leadings from one capstone legislation to another. Such an approach overemphasizes the role of formal laws in triggering social and institutional change. In reality, legislation itself does not cause change; its scope, significance, and relevance are determined in the process of interpretation and further implementation. To understand when, where, and how change happens and what role the law plays in the process, we need to look at local phenomena and on-the-ground practices. The event-based perspective fails to recognize that the passage of legislation is the continuation of a process that begins before and lasts after its adoption . The legal prohibition of cannabis is not merely a political project organized by a cohesive group of elite actors but a multilevel, complex, and dynamic social process. Formal legislations affirm rather than cause social and cultural changes, especially when the primary function of law is symbolic. For example, the legalization of the medical use of cannabis in California in 1996 did not affect cannabis arrest rates. The number of arrests for cannabis possession was steadily growing until 2010 and decreased five-fold in one year after the passage of SB-1449, which reclassified the possession of small amounts of cannabis as a misdemeanor . Thus, the legalization of medical cannabis symbolized the public affirmation of new social norms and ideals, but it did not change law enforcement practices. The SB-1449, on the contrary, affected people’s behavior in a more direct and instrumental manner, but its adoption would not be thinkable without the preceding cultural shift. On Fig. 1, the legalization of the medical use of cannabis in California would be a constructed event, while the adoption of the SB-1449 would be on the level of observable occurrences that followed the event.

There is a broader acceptance of cannabis in the mainstream media and among the public

Nevertheless, it would be erroneous to assume that acceptance of cannabis arose with the same intensity across California counties and cities.At the moment, only one-third of California cities permit the distribution, cultivation, testing, manufacturing, or sale of cannabis, while the rest have passed ordinances forbidding any cannabis-related economic activities within city borders. This project is the first and the most comprehensive study of the unfolding process of cannabis legalization, which empirically addresses a set of interrelated questions. First, how is the legalization of cannabis for recreational use spreading across California cities? Second, what accounts for the uneven legalization of cannabis across California cities? And third, what does the case of cannabis legalization reveal about the relationship between legitimacy and legality more generally? Historically, the war on cannabis drew a line between “productive” members of society and “dangerous” elements , thereby serving normality judgments. Today, cannabis is in a transition state: although the law made it legal in California, large segments of the population and local governments still do not accept the idea of recreational cannabis and refuse its incorporation in the communities. I explore cannabis regulation as an example of moral laws, i.e., policies that deal with problems of moral order and deviance . According to Max Weber, certain laws, especially those relating to moral issues, are not accepted on the basis of their legality, but because they express legitimate moral values . The legalization of cannabis in California presents an ideal context to unpack the mechanisms that explain why some jurisdictions move toward more permissive moral policies, and others do not. The implementation of morality policies at the city level is a neglected area of research. Many writings focus on the nature of morality policies and whether or not the state should regulate certain individual practices. Still, little efforts have been directed toward understanding how these legislations are created and carried out. This study provides an empirical test of the morality of law and explains what drives the adoption of permissive cannabis regulation at the bottom level of US politics—cities.

There is a commonly held misconception that since most citizens supported the legalization of cannabis in California,vertical agriculture its use and sale is allowed throughout the state. However, a closer look at local political projects shows that public support cannot fully explain the adoption of pro-cannabis legislation. For example, in Santa Monica, 75 % of voters supported cannabis legalization in 2016, but the city government forbade all economic activities related to recreational cannabis. Meanwhile, most of Firebaugh’s citizens voted against the legalization of recreational cannabis, but the city government permitted cannabis businesses. By focusing on cannabis legalization in the cities of California, this project brings to the fore the importance of local actors and decisions. Despite the significance of local policies, we still lack a comprehensive body of theoretically driven research explaining variation in policy outcomes at the local level . Studying local policies can be beneficial for many reasons. First, the success or failure of state or federal regulations depends on how it is operationalized and put into effect by local jurisdictions . Local practices may change an idea laid by legislators. Second, the decisions produced by local governments have a direct impact on the well being of citizens and communities. Lastly, a large number of national initiatives have grown out of local activities . For example, San Francisco was the first American city that ignored state and federal laws and decided not to prosecute “underground cannabis pharmacies”, which eventually paved the way for legalizing cannabis across the state . This research delves into the complex nature of change. I purposely do not say “legal change”, for the term oversimplifies the dynamics inherent to the transition from illegal to legal cannabis. Legalization is not a mere outcome and does not happen overnight. It is, first of all, a gradual process of social, cultural, legal, and institutional transformation, which started in California well before Proposition 64 in 2016 and may extend long into the future. Therefore, I adopt a process-oriented approach that overcomes the limitations of the outcome-based perspective, widely used in sociolegal studies, and allows us to speak about legalizing cannabis in California as a project under construction. Socio-legal scholars examine the drug problem predominantly through the lens of control, prohibition, and punishment. Drugs are viewed as a card played within the bigger political project that is the creation of the penal state. Various studies have demonstrated how drugs became both a target and a source of the war on crime, and how by associating drug use to violent crimes, the federal government made the war on drugs an integral part of American life . These analyses present a causal explanation of penal change, which contains the following key elements: the intensification of state control as a response to the public’s fears incited by the mass media. Although such theorizing is essential for understanding the drug problem, it is not sufficient.

The preponderance of the “crime and punishment” framework stifles other possible research angles and reduces the perimeter of the drug problem. Drugs are not only a criminal justice issue but also a societal problem, a medical problem, a moral problem, a market problem, and so on. Another limitation of the existing socio-legal literature on the drug problem is its outcome based and top-down orientation. The research heavily relies on causal explanations and focuses on macro-level trends, elite political actors, class conflicts, federal policies, and national mass media. At the same time, it neglects other layers of interpretation, such as local-level processes, institutional practices, and social relations. As Andrew Abbott notes, the vast majority of sociological studies aim to evaluate the causes of “what happens” but lack a reflective concept of how to temporarily conceptualize “what happens” . Instead of focusing on where we ended up, Abbott says, we should look at the walk itself. The outcome-based theorizing may be problematic since it reinforces a “normal” and “objective” view of life . By explicating the causal relationship between crime and drugs, socio-legal scholars conceptualize the drug problem in the same inadequate way as policymakers and therefore run the risk of exacerbating intolerance and prejudices . Currently, socio-legal scholarship lacks an alternative language to speak about the drug problem and continues investing in the criminal justice approach . Cannabis legalization raises interest among various social science disciplines—jurisprudence, sociology, criminology, political science—which focus on a narrow range of topics, such as the discrepancy between federal and state regulation, or the effect of legalization on crime rates, drug use, and driving under the influence. Much of this scholarship defines law in instrumental terms: legal change simply ensues from broader policy and regulatory shifts, such as the passage of the Controlled Substance Act in 1970 or Proposition 64 in 2016. However, the law is not a direct reflection of collective consciousness; neither is it the immediate result of lawyers’ work. The alternative way to study law is to look at it as a communicative practice that directs attention to the law’s power in constructing meanings, legal discourses, symbols, interpretations, and knowledge. In short, to understand the nature of the legalization process, it is necessary to employ a bottom-up perspective and move from the enactment of federal and state legislation to local political projects, from the patent outcomes to latent everyday practices, and from the direct impacts to negotiated agreements.Many intricacies of the legalization process become apparent only when we apply a processual view and focus on local political projects.

What do we mean when we say that cannabis became legal in California? From a strictly juridical point of view, it means that possession, cultivation, distribution, and sale of cannabis do not represent a criminal act anymore. Individuals who consume, grow, or sell cannabis cannot be arrested or prosecuted. From a market point of view, it means that the state oversees the operation of the legal cannabis market. Namely, it creates a legal infrastructure for market development, enforces contracts, safeguards competition, protects property rights, and provides standards. Finally,hydroponics flood table from a cultural perspective, legalization means a cultural shift reflecting the broader public acceptance of cannabis. Some scholars call this process “normalization” , arguing that today, cannabis use is more tolerated, and cannabis users are less stigmatized. But how do all these processes evolve in practice? First, even though de jure cannabis is legal throughout California, de facto its status is controversial. Under the Controlled Substances Act , cannabis is classified as a Schedule I drug, along with such potent substances as heroin and LSD. Thereby, the federal government has the authority to prohibit and prosecute any use of cannabis. As separate sovereigns, the states may decide whether to cooperate with the federal government or not, but they cannot prevent the federal agencies from enforcing the law. Therefore, cannabis is legal in a state that has voted to allow it, but only to the extent the federal government chooses not to enforce the CSA . Despite several attempts to resolve the conflict between federal and state laws, cannabis is still in a legal limbo. In 2013, the Department of Justice issued a memo notifying that prosecuting local cannabis cases is not a priority.3 However, under the Trump administration, US Attorney General Jeff Sessions rescinded all Obama-era lenient policies towards cannabis, including the memo limiting federal prosecution of local cannabis cases.4 Currently, Congress is debating over the Marijuana Opportunity and Reinvestment and Expungement Act , which decriminalizes cannabis and completely removes it from the list of controlled substances.5 On December 4, 2020, the House of Representatives approved the ACT, but many experts are pessimistic about its passing in the Senate. Second, the continued status of marijuana as a federally prohibited substance significantly hampers the states’ capacity to implement new regulatory policies effectively and creates legal jeopardy for those in the legal cannabis market .

As Sam Kamin notes, “federal prohibition acts as a brake on an industry that otherwise might grow with unhealthy pace” . Cannabis dispensaries cannot obtain banking services since financial institutions are not ready to support companies selling a product that the federal government treats as an illegal drug. They have to rely mostly on cash and thus become an easy target for robberies . Additional obstacles for legal cannabis companies are associated with high taxes, difficulties with obtaining legal aid, and unavailability of property rights protection and other business necessities . The adversity of the business environment induces some cannabis distributors to stay outside of the legal market. In 2018, California legal dispensaries sold fewer products than a year before, when only medical cannabis was allowed6 , a picture which many specialists associated with the persistence of illegal or semi-legal economic activities.7 Third, in the last decades, cannabis use has undergone a transition from a largely marginal activity to a more prevalent and tolerated one . California issued more than 20,000 cannabis licenses within the first two years of the legalization of recreational cannabis. The number of professional associations, networks, business-related newspapers, websites, and the variety of cannabis products is continually growing.According to Gallup, the support of cannabis legalization grew from 12% in 1969 to 66% in 2018.8 After the possession of less than an ounce of cannabis was reclassified as a misdemeanor9 in 2010, the number of arrests for cannabis possession has dropped considerably—from 56,000 in 2010 to 10,000 in 2011 . The evidence indicates that cultural attitudes to cannabis have changed. However, there is still a certain stigma associated with cannabis. Despite its normalization, cannabis use continues to be perceived as risky, marginal, and deviant and is often kept private to escape conflicts with family, landlords, employers, or police . The war on drugs generated various misconceptions about cannabis, which detrimentally affect the image of current cannabis users and cannabis dispensaries. For example, under Proposition 64, public and private companies have a right to ask job candidates and current employees to pass a test on cannabis to “maintain a drug and alcohol-free workplace.” If a drug test shows traces of cannabis, a person can lose a job. 10The legal status and the meaning of specific products vary over time and space .

Several studies have analyzed cannabis use among pregnant and non-pregnant women

One other study has utilized results from TAG to predict cannabis involvement in an independent sample. Vink and colleagues used polygenic scores created by aggregating SNPs at varying p-value thresholds, up to p = 0.5, to predict tobacco, alcohol and cannabis phenotypes in an independent Dutch sample. Such an approach has the advantage of utilizing effects, even those that are not statistically significant, to create a genetic predictor that is more consistent with the polygenic view of complex behavioral traits. The TAG polygenic scores for CPD were associated with CPD and cannabis use but the extent to which the most significant SNPs contributed to these findings is unclear. Our analyses studied these highly significant individual SNPs that were part of the polygenic scores and while we note a similar degree of association with rs1051730, we do not see any association with other TAG SNPs . We also extended the association to cannabis abuse/dependence, a phenotype that was not studied by Vink et al. . It is also worth noting that, for both tobacco smoking and cannabis involvement, SNPs associated with initiation were not associated with later stages of CPD or abuse/dependence. Prior twin analyses have suggested substantial overlap across genetic factors influencing initiation and problem use of both tobacco and cannabis . Nonetheless, evidence for genetic factors that only influence later stages of tobacco dependence have also been identified and is consistent with our results and those from the prior smoking meta-analyses . Some limitations of this study are worth noting. First, the evaluations focused only on European American subjects as the meta-analyses were restricted to similar samples. Second, these data did not have a quantitative “CPD-like” measure of cannabis use. Third, as the sample was enriched for alcohol dependence, rates of cigarette smoking and cannabis involvement are higher than those noted in the general population. This enrichment, particularly as one of the contributing samples was ascertained for nicotine dependence , may have influenced our ability to detect these associations. Importantly,grow rack the associations with cannabis involvement should be viewed as preliminary and pending replication.

In conclusion, while analyses revealed some promising relationships of SNPs previously implicated in tobacco smoking with cannabis measures, these associations did not appear to be independent of their effects on smoking. As large meta-analyses of tobacco smoking have shown, by increasing sample sizes, common variants associated with cannabis involvement can also be successfully identified. Such discoveries will enhance our ability to identify such cross-disorder SNPs with greater precision, as has been witnessed for phenotypes such as schizophrenia, bipolar disorder, major depressive disorder and autism .Previous literature shows a link between the passage of laws legalizing the use of cannabis for medical or recreational purposes and the possible effect on use rates, especially among youth. However, research into the impact of cannabis laws among pregnant women is limited. This study finds consistent associations of legalization of recreational cannabis is associated with cannabis use throughout the reproductive cycle; medical cannabis legalization was also associated with cannabis use, but only in the preconception period.These findings suggested legalization may play a role in cannabis use during pregnancy and providers may want to consider how and when the council pregnant women about substance use in states with legalization.In 1996, California became the first state to legalize cannabis for medical use and 28 other states have since followed suit, with eight also allowing recreational cannabis use . Today, cannabis is the most used illicit drug in the past year among all adults; 10–34% report use and among pregnant women 4–9% of report use . Cannabis use during pregnancy may have adverse effects on perinatal and child health outcomes including low infant birth weight and child mental health concerns . Moreover, the perception of harm of cannabis use among pregnant women may be decreasing along with legalization, given that some dispensaries “recommend” cannabis to pregnant women to alleviate pregnancy-related symptoms . Additionally, studies show that women who use cannabis during pregnancy are also more likely to use it in conjunction with tobacco, the most used substance during pregnancy.The concurrent use of two substances is a potentially dangerous combination since tobacco and cannabis use together is associated with an increased risk of adverse birth outcomes including stillbirth and small-for-gestational age when compared to using one substance alone.Researchers reported that low education levels, being unmarried, and using other substances all correlate with cannabis use, and very few studies examine state legalization status as a potential factor.

Studies of the general population and the limited studies including pregnant women show that cannabis legalization may affect patterns of substance use behavior and in recreationally legal states show a direct correlation between cannabis legalization and increased use of cannabis . Studies of pregnant women specifically found, those who use cannabis tend to enter prenatal care later and to use other substances along with cannabis, including tobacco . Importantly, these studies did not account for electronic cigarette use, even though studies show known health risks and use has increased markedly in the U.S. among pregnant women . Therefore, studies are needed that account for e-cigarette use when evaluating prenatal and postnatal concurrent tobacco and cannabis use. Furthermore, given the evolving cannabis policy in the U.S. and negative health impacts of both cannabis and tobacco on fetal and women’s health, current data specific to pregnant women that also account for state legalization status are crucial for advancing prenatal care and education. The current study was designed to address these gaps. Using the Pregnancy Risk Assessment Monitoring System survey, the sample included respondents from 16 states with varying types of cannabis legalization to: Examine the prevalence of cannabis use during the preconception, prenatal and postpartum periods Determine prevalence of co-occurring use of tobacco before and during pregnancy Identify factors associated with preconception, prenatal, and postpartum use of cannabis including state legalization status. We hypothesized that factors associated with cannabis use during the three time periods will be like those in the general population including a higher use among women living in states with recreational cannabis legalization. Additionally, we predicted women using cannabis would be more likely to co-use other substances, particularly, tobacco.The study is a secondary analysis of cross-sectional survey data collected in the 2016 through 2018 Pregnancy Risk Assessment Monitoring System by the Centers for Disease Control and Prevention and state health departments. Based on a stratifed sampling frame which selects from recent live births with complete birth certifcate data, the PRAMS survey is sent to pregnant women six weeks to three months post-partum throughout the United States as a way of monitoring perinatal health behaviors and experiences and their subsequent impact on infant health outcomes.

Comparison between states is possible through standardization of procedures and survey instruments. The sample for the current study included women 18 and older who provided an answer to the questions about cannabis use. Women under 18 were excluded due to the potential confounding factors associated with adolescent pregnancy and their lack of legal access to cannabis. The fnal sample included 36,391 women living in 16 states in the United States who were administered questions specific to cannabis use on the PRAMS survey and who gave birth between January 1st 2016 and December 31st 2018 for a weighted sample reflecting 15,486,000 women. Given the study design and possibility that women answered the survey for multiple pregnancies, prior to analysis we searched for duplicate participant identifiers and did not find repeat ids. Women agreed to participate in the survey with the knowledge and consent that the data may be used for scientific study . The study was reviewed and deemed exempt from further review by the University Institutional Review Board based on use of deidentified data. Therefore, the study was completed in accordance with the ethical standards in the 1964 Declaration of Helsinki and its later amendments.Individuals were classified either as cannabis users if they answered “yes” or nonusers if they answered “no” during three time periods: before pregnancy , during the last three months of pregnancy , and 6–12 weeks after delivery . Three prevalence outcomes were examined. Cannabis use during the last three months of pregnancy based on the question, “At any time during the last 3 months of your recent pregnancy, did you use marijuana or hash in any form”?; Cannabis use in the 12 months before pregnancy , based on the question, “At any time during the 12 months before your recent pregnancy, did you use marijuana or hash in any form”?; and cannabis use since delivery based on the question, “At any time during the 3 months since delivery,greenhouse grow tables did you use marijuana or hash in any form”? The final question regarding use after delivery was only asked in 8 of the 16 states analyzed .All analyses were conducted using Stata version 14 with PRAMS weights applied to account for the complex sampling design and generate estimates generalizable to pregnant women across the United States.

The PRAMS weights are determined by multiplying the sampling, non-response, and non-coverage of the weight yields and more detailed is available elsewhere . Less than 1% of respondents were missing data for any covariate; therefore, variables with missing data were imputed with the mode. All analysis was repeated with complete cases only with no substantive differences in the findings; results presented include imputed values. The prevalence of cannabis use was estimated in each of the three time periods . All socio-demographic and prenatal care characteristics were summarized and compared with chi-square tests for cannabis users and non-users during each time . Logistic regression was used to examine each cannabis use outcome as a function of state cannabis legalization status and the socio-demographic and health covariates. As a sensitivity analysis the three models were also repeated with clustering at the state-level, with no substantive differences to the reported results.In a large sample of pregnant women in 16 states, we contribute estimates of the prevalence of cannabis use in the preconception, prenatal and post-partum periods finding associations with use in recreationally legal states and some associations with residence in medically legal states. The rate of cannabis use before pregnancy reported here is consistent with nationally reported rates of 10.5% of the general population using cannabis but lower than recent rates of 34% in 2020, reported by the National Institute on Drug Abuse . To date, few studies include both recreational and medical cannabis legalization status as factors potentially influencing perinatal cannabis use.Studies that include legalization were typically limited to only one state and only addressed recreational legalization.Several factors could account for the state legalization status and association with use found in the study. First, as states legalize cannabis, women’s perception of harm of cannabis decreases, resulting in an increase in use rates as noted in the general population . To support this point, findings from a qualitative study where in-depth interviews were conducted found that 62% of women using cannabis reported that they would increase their use during pregnancy if cannabis was legalized . Similarly, another study found that women who used cannabis during pregnancy did not believe the substance harmful . Second, the opening of dispensaries following state legalization allows for better access to and promotion of cannabis use, possibly leading to use during pregnancy. In Colorado, a study of dispensaries found 69% recommended cannabis to the researcher who was claiming to be pregnant and asking for a recommendation on the use of cannabis during pregnancy . Future studies are needed to test the proposed mechanisms as drivers of use among pregnant women in recreationally legal states. The finding in this study of a higher odds of using cannabis during the preconception period in recreationally legal states and medically legal states are of particular concern given 45% of pregnancies in the United States are unplanned . In the cases of unplanned pregnancy, women using cannabis could unknowingly expose the embryo to cannabis derivatives like tetrahydrocannabinol during a critical period of fetal development. However, given that the preconception data for this study were collected up to twelve months before pregnancy to the study cannot accurately measure how close to conception women were using cannabis.

The minimum capital investment in testing equipment needed to satisfy regulations is substantial

To complement licensed testing lab data, we also drew on personal interviews, phone calls, and email exchanges with sales representatives of three large equipment suppliers. Table 3 summarizes capital costs, other one-time expenses, and annual operational and maintenance costs used in our calculations. We report average cost and standard deviation for each estimate. We assume that medium-sized and large labs receive discounted prices on equipment, given the larger scale of their purchases. Based on information provided by equipment suppliers, we expect these discounts to be between 1.5% and 2.5%. Different-sized labs have different capacities based on their scale. We assume that larger labs have made larger capital investments and are better able to optimize processes when supplying a larger volume of testing . On the other hand, small testing labs require less equipment and less capital investment, and operate with low annual costs, but their testing capacities are also low. Table 4 summarizes our estimates of running time for tests, the main consumables used by testing machines, and the expected cost of running a specific test per sample. In addition, we include a range of $80 to $120 per sample to cover general material and labor apparel used while preparing and processing samples. We estimate that in small labs , capital investment in equipment is about $1.1 million; in the medium-sized labs , capital investment in equipment is about $1.8 million; and in large-scale labs , capital investment in equipment is about $2.8 million. These capital costs,grow rack system amortized over a 10-year time span with a 7.5% rate of depreciation and interest, represent less than 15% of total annual expenses.

Annual costs of operating range from $1.4 to $2.2 million for small labs, $2.7 to $3.7 million for medium-sized labs, and $6.2 to $8.1 million for large labs. Consumables are the largest share of total annual costs in large-scale labs, whereas labor is the largest share of costs in small-scale labs. In medium-scale labs, consumables and labor have about equal shares of annual costs. Different-sized labs differ in their capacity and efficiency. The highest possible sampling cost we assume for small labs is about $35 per sample if the distributor is located 156 miles away . On average, costs of collection, handling, and transportation represents a small share of total lab costs per sample. Fig 4 shows the distribution of full testing cost per sample from 1,000 Monte Carlo simulations assuming 49 labs. Variability of the cost per sample within small labs is high, with the highest and lowest cost within that group differing by $463. The difference between the highest and lowest costs in large labs is $88, with a lowest cost per sample of about $273. The average full cost per sample tested is about $313 for large labs, $537 for medium labs, and about $778 for small labs . Large cost differences per test and per batch document the large-scale economies and differences in operational efficiencies across labs of difference sizes. The aggregate amount of cannabis flowing through licensed labs in 2019 remains relatively small relative to the anticipated amounts expected in the future. That means labs that may anticipate growth, operate well below capacity. Substantial scale economies suggest that,grow rack system as the market settles, the smallest labs must either expand to use their capital investment more fully, leave the industry, or provide some specialized services to distributors that are not accounted for in the analysis presented here.

Simply put, the average cost differences shown in Table 6 or the simulated ranges displayed in Fig 4 should not be understood as a long run equilibrium in the cannabis testing laboratory industry.Based on the shares developed based on current information, a few large labs are likely to supply almost half the testing services for cannabis sold through licensed retailers in California, while medium labs will test about 24% of cannabis and small labs about 30% of cannabis. Using these shares and the cost information documented, the weighted average of testing cost from our simulations is about $504 per sample.In 2018, the first year of mandatory testing enforcement, according to official data published by the California Bureau of Cannabis Control and posted publicly on its website, failure rates in California averaged about 5.6% . Failure rates for the first seven months of 2019, the second year of the testing regime, have averaged 4.1%. We assume a 4% failure rate for the current market in California. By comparison, in Washington State, in 2017, the second year after the testing began, 8% of the total samples failed one or more tests. The Colorado Marijuana Enforcement Division reported that during the first six months of 2018, 8.9% of batches of adult-use cannabis failed testing, with infused edibles and microbial tests for flower accounting for the most failures. Batch size significantly affects the per-pound testing cost of cannabis marketed, especially when batch size is smaller than 10 pounds. Fig 5 shows the costs of one pound of cannabis marketed coming from different sizes of batch flowers using 0%, 4%, and 8% rejection rates. As rejection rates increase, the differences between the costs per pound of testing different batch sizes decreases. For example, given a 0% rejection rate, the cost of testing per pound of cannabis marketed from a one-pound batch is about 27 times higher than the cost of a 48-pound batch; on the other hand, given an 8% rejection rate, the cost of testing per pound of cannabis marketed from a one-pound batch size is only seven times higher than the cost from a 48-pound batch size.Table 7 shows costs per pound of cannabis testing itemized into laboratory cost, the value of lost inventory, and the cost of remediating failed batches, given different rejection rates and batch sizes. For small batch sizes, laboratory costs are a higher share of total testing costs than they are for large batch sizes. For a one-pound batch size, the total cost of testing of a pound of cannabis that reaches the market is about $641 when the expected rejection rate is equal to zero. The cost increases to $714 if the expected rejection rate is equivalent to 4%, and to $791 if the expected rejection rate is 8% . The share of laboratory cost from total cost decreases as the rejection rate increases and the value of lost inventory therefore increases.

Under an 8% expected rejection rate, the share of lost inventory is half of the total cost for eight-pound batches .In this paper, we use a simulation model to estimate the costs per pound of mandatory cannabis testing in California. To do this, we make assumptions about the cost structure and estimated the testing capabilities of labs in three different size categories, based on information collected from market participants across the supply chain. For each lab, we estimate testing cost per sample and its share, based on testing capacity, of California’s overall testing supply. We then estimate a weighted average of the cost per sample and translate that value into the cost per pound of cannabis that reaches the market.We use data-based assumptions about expected rejection rates in the first and second round of testing, pre-testing, and the remediation or processing of samples that fail testing. Our simulations rely on information collected from several sources, including direct information from testing labs in California, price quotes from companies that supply testing equipment,rolling flood tables interviews with cannabis testing experts, data on testing outcomes for cannabis and other agricultural products from California and other states, data on pesticide detection in California crops, and data on average wholesale cannabis batch sizes.As lab scale rises, testing capacity rises faster than do input costs, so average costs fall with scale. We find that a large lab has four times the total costs of a small lab but 10 times the testing capacity, in part because large labs are able to use their resources more efficiently. Testing cost per pound of cannabis marketed is particularly sensitive to batch size,rolling flood tables especially for batch sizes under 10 pounds. Testing labs report that batch size varies widely. The maximum batch size allowed in California is 50 pounds, but many batches are smaller than 15 pounds. We assume an eight-pound average batch size in the 2019 California market, but we expect that the average batch size will increase in the future as cultivators become larger and more efficient and take advantage of the opportunity to save on testing costs .Testing itself is costly, but losses inflicted by destroying cannabis that fails testing is a major component of overall costs. Low or zero tolerance levels for pesticide residues are the most demanding requirement, and result in the greatest share of safety compliance testing failures. Cannabis standards are very tight compared to those for food products in California. A significant share of tested samples from California crops have pesticide residues that would be over the tolerance levels established for California cannabis . Some foods that meet pesticide tolerance established by California EPA may be combined with dried cannabis flowers to generate processed cannabis products . Pesticide residues coming from the food inputs may generate detection levels of pesticide over the tolerance levels set by cannabis law and regulation, even if they are otherwise compliant as food products.

Cannabis testing regulation is strict compared to tobacco, another inhalable crop. Tobacco has no pesticide tolerance limits because it is considered to be an inedible crop used for recreational purposes.Cannabis has multiple pathways of intake, such as edibles, inhalable, patches, etc., and also may be prescribed for people with a health condition, searching for alternatives to traditional medicine. Some labs report that when samples barely fail one test, they have a policy of re-testing that sample to reduce the probability of false positives. Some labs have reported up to 10% in variation in test results from the same sample. Some labs indicate that about 25% of samples need to be re-tested to be sure that results are accurate. Such concerns have been widely reported. In July 2018, some producers voluntarily recalled cannabis products after receiving inconsistent results of contaminant residues from different laboratories; and some California labs have also been sanctioned by the Bureau of Cannabis Control for failing state audits on pesticide residue tests. A major issue for legal, taxed and licensed cannabis market is competition with cannabis marketed through untaxed and unlicensed segment. Higher testing costs translate into higher prices in the licensed segment. Safety regulations and testing may improve the perceived safety and quality of cannabis in the licensed segment, thus adding value for some consumers. However, price-sensitive consumers move to the unlicensed segment when licensed cannabis gets too expensive. A useful avenue for further research is to investigate cannabis testing regulations and standards across states to assess implications for consumer and community well being and competition with unlicensed cannabis. Compared with other agricultural and food industries, the licensed cannabis industry in California has relatively little data. Banking is still done in cash, and sources of government financial data are less available for cannabis than they are for other industries. As the licensed cannabis segment develops, we expect that increased access to data on the market for testing services, including on prices, quantities, and batch sizes. Data from tax authorities, the track and-trace system, and the licensing system will then help clarify the costs and implications of mandatory cannabis testing.Assessing the environmental impacts of the cannabis industry in Northern California has been notoriously difficult . The federally illegal status of cannabis has prevented researchers from obtaining funding and authorization to study cultivation practices . Fear of federal enforcement has also driven the industry into one of the most sparsely populated and rugged regions of the state , further limiting opportunities for research. The result has been a shortage of data on cultivation practices and their environmental risks . An improved understanding of cannabis cultivators’ water use practices is a particularly pressing need. Given the propensity of cannabis growers to establish farms in small, upper watersheds, where streams that support salmonids and other sensitive species are vulnerable to de watering , significant concerns have been raised over the potential impacts of diverting surface water for cannabis cultivation.

We hypothesized that cannabis exposures would increase significantly following each of these changes, especially among children

Marked increases in cannabis sales were reported by industry and business sources during the COVID-19 pandemic,1 and studies of cannabis users in the U.S.,2 the Netherlands,and Canada reported increased cannabis use during the pandemic. Content analyses of Twitter and other social media and online sources during the pandemic found increased discussion of cannabis as treatment for COVID-19. The number of cannabis dispensaries is increasing with legalization and dispensaries are an important source of marketing messages,but it is unknown to what extent online cannabis dispensary marketing activities may have contributed to increased pandemic sales. We analyzed cannabis dispensary websites to gain insight into cannabis marketing practices at the start of the COVID-19 pandemic, leveraging an existing study of a small geographic area with two different historical policy contexts. The region from the San Francisco/Bay Area extending east to the Reno area of Nevada is readily accessible by car, but includes two states with contrasting regulatory contexts: California has a long history of acceptance of cannabis including the counterculture of the 1960-1970s, medical marijuana legalization in 1996, and a longstanding cannabis cultivation.In contrast, Nevada’s medical marijuana policy was implemented fairly recently in 2015. Both states approved adult use cannabis in 2016, and deemed cannabis dispensaries essential during the pandemic. Dispensaries remained open in California; in Nevada, dispensaries were initially limited to delivery, but in May 2020 allowed curbside pick-up and in-store purchases.An August 2020 cannabis industry report found that sales rose overall during the COVID-19 pandemic, and stated that “e-commerce solutions were the key to success”.As part of an ongoing surveillance study comparing dispensary websites in San Francisco, Alameda and Washoe Counties, we noted special COVID-19 announcements appeared days to weeks following lockdown and analyzed these to gain insight into industry marketing responses to the pandemic.

We conducted a content analysis of website announcements, comparing dispensaries in San Francisco/Alameda Counties and those within a 30 mile radius of Reno, Nevada, between April-May 2020. We obtained lists of all licensed dispensaries from local health departments and verified the businesses were open using online resources . We included all dispensaries with functional websites . Three trained researchers reviewed all websites and recorded all content related to COVID-19 with screenshots. COVID-19 announcements appeared on websites shortly after the stay-at-home orders were announced, often as pop-up windows appearing on top of the home page, or as an extra web page. We coded only content from the dispensary, not unrelated pop-up ads. The content code book was adapted from a prior website content analysis instrument,and the team iteratively reviewed, discussed,best trimming trays and revised codes, including six new codes specific to the COVID-19 pandemic . Two authors double coded all COVID-19 announcements for the presence of each theme; the unit of analysis was the website. The team met weekly to review and discuss discrepancies in coding and to resolve conflicts, using a fourth investigator to adjudicate if needed. Inter rater reliability measured by Krippendorff’s alpha was 0.77 – 1.0 for COVID-19 variables. COVID-19 announcements were present on 25/32 of dispensary websites in San Francisco/Alameda counties and on 9/15 of websites in the Reno area. Of the websites with COVID-19 content, almost all announced operational changes.72% of San Francisco/Alameda and 56% of Reno websites announced safety measures like wearing masks, hand washing, cleaning surfaces, temperature checks, physical distancing, notouch payment or eliminating smell samples. Some asked patrons not to come to the dispensary if feeling ill, with reference to sick or immuno compromised clients. The majority of websites made references to, or provided links to, government or public health resources, either as the explanation for changes in operations, or to demonstrate that the dispensary was cooperating with health authorities. The look and feel of COVID-19 website announcements varied widely; most dispensaries utilized simple “pop up” windows on the site home page with text announcements to customers of altered hours, access or pick up policies. However a few created custom graphics for promotions or discounts, and one dispensary adorned their logo with a mask. Health-related announcements were present in several forms. Of San Francisco/Alameda County dispensary websites, 36% referred to their status as an essential service, a theme not found on Reno area websites. The status as an essential service was often accompanied by references to cannabis as a medicine, or a connection to health authorities. For example, one San Francisco dispensary posted a quote “Cannabis is an essential medicine for many San Francisco residents.

Dispensaries can continue to operate as essential businesses during this time, while practicing social distancing and other public health recommendations” that was attributed to the San Francisco Department of Health. Another announcement stated, “So, what are the essentials? Groceries, prescription drugs, gas, urgent medical care, and weed. This list is what’s necessary for the health and safety of us all during this time.” More frequently, COVID-19 announcements included more general health statements such as, “The health and safety of our staff and customers is of the utmost importance to us.” A few dispensaries gave advice about ways to use cannabis, implicitly to avoid COVID-19 infection, such as, “Opt for non-invasive forms of ingestion – edibles, tinctures, drinks, soft gels” and “Do not share cannabis joints, vapes or edibles.” One dispensary suggested cannabis use for anxiety, “If you are experiencing anxiety, consider using products with CBD, THCA, CBN, or low doses of THC” and this dispensary also suggested bulk purchases as a way to mitigate COVID-19 infection risk, “Limit your need to travel by stocking up on your supply. We are offering a 10% discount when you buy 4 eighths or 4 extracts , stackable with our senior and veteran discounts.” 44% of Reno websites included general statements about the importance of their patrons’ health, while general statements were found on only 16% of Bay Area websites. However, all announcements that recommended specific ways to use cannabis to mitigate COVID-19 risk or anxiety were from Bay Area dispensaries. One Bay Area dispensary asked patrons to contact local county supervisors to advocate for cannabis as an essential service and provided links. A minority of sites in San Francisco/Alameda Counties offered COVID-19 discounts or specials, including a “heroes discount” for health workers, and discounts for those laid-off from work. One San Francisco dispensary sold surgical masks and hand sanitizer. This is, to the best of our knowledge, the first study describing the novel COVID-19- related marketing communications from cannabis dispensaries during stay-at-home orders. We found that like other businesses, most cannabis dispensaries implemented operational changes, including delivery, pickup, and sanitation measures and made general statements that health and safety was important to them. In addition, however, we found some announcements on dispensary websites that were more unique to cannabis sales, such as those that linked cannabis and health, either by referring to cannabis as medicine, making references to government or health authorities, and utilizing rhetoric similar to healthcare providers, including making recommendations for cannabis forms to reduce disease transmission risk or strains to use if one was experiencing anxiety. The study’s strength is that it provides a novel and timely snapshot of practices that may influence cannabis consumption in a rapidly evolving context. Surveillance of cannabis websites can complement and help to explain findings of the behavioral studies reporting increased cannabis use. This aspect of cannabis marketing may be more agile and difficult to capture with traditional marketing surveillance; the COVID-19 announcements appeared soon after lock downs. By October 2021, all of these announcements had disappeared from all but one of the dispensary websites . This study captured a unique transient marketing activity, suggesting that during future societal events or stressors that might impact cannabis consumption, rapid surveillance of cannabis dispensary websites is warranted.

The study has several limitations: it has a small sample size, this cross-sectional analysis of dispensary websites does not represent other types of cannabis websites or places without legal cannabis,trimming tray and it addresses only dispensary COVID-19 announcements, not consumer behavior. This study adds perspectives on new COVID-19 related content to prior analyses of cannabis advertising,which also found retailers positioning themselves as healthcare providers. While we did not find explicit claims that cannabis could treat COVID-19, some announcements suggested ways to use cannabis to avoid infection, or suggested cannabis products to use if experiencing anxiety; these implicit connections between cannabis and health are consistent with prior studies finding cannabis dispensary health benefit claims.This analysis was limited only to the COVID-19 announcements; a subsequent analysis of the full website content found that anxiety was the most common mental health claim, present on 80% of websites in this sample.This limited analysis of the COVID-19 announcements from a single time point early in the pandemic likely underestimates the potential influence dispensary marketing could have on cannabis consumption. While COVID-19 announcements had largely disappeared from dispensary websites by October 2021, an informal review of the dispensary websites included in this analysis in October 2021 found that one featured a blog post on the topic, “Can marijuana cure coronavirus?” and several websites featured blog posts about using cannabis for anxiety, a topic indirectly relevant to the stress of the ongoing pandemic. While this study does not address consumer behavior, another study found in states where cannabis is legally sold, people with mood or anxiety disorders were more likely to use cannabis to selfmedicate.The marketing tactics we observed to maintain cannabis availability and align with health authorities might contribute to increased cannabis use, particularly since other studies show stress and decreased access to medical services were associated with the pandemic. This study demonstrates that cannabis dispensary websites are a timely source of data on industry responses to rapidly changing events and public health policies that may impact cannabis consumption.

The study identified two tactics in the COVID-19 announcements preserved which might contribute to increased cannabis consumption: preserving ready access to cannabis as an essential service, and reinforcing perceptions of cannabis as a medicine and dispensaries as health services. While use of online ordering and delivery services has been documented in prior analyses of cannabis websites we found these services became nearly universal after stay-at-home orders. The increase in home delivery of cannabis and other intoxicants that accompanied the COVID-19 pandemic may persist, posing new challenges for surveillance research.Future studies should address the impact of cannabis marketing and messaging on consumer cannabis consumption and health-care seeking behavior during stressful events. In 1996, California became the first state to legalize the use of medical cannabis. Children’s exposures to cannabis increased by a factor of three from 2010 to 2016. For children under 6 these exposures were primarily accidental; for adolescents these exposures were more commonly related to cannabis misuse. In November 2016, two decades after the medical legalization, Californians passed a ballot measure legalizing recreational cannabis. Recreational cannabis legalization has been associated with increased exposures, and these disproportionately affect vulnerable groups, particularly children, young adults, and older adults. As of 2021, eighteen states and the District of Columbia had legalized recreational cannabis and cannabis had been decriminalized in 27 states. In the first two years after recreational legalization in Oregon and Alaska, local poison control centers reported increased accidental cannabis exposures among children, commonly leading to sedation. Children were also more likely to be admitted to intensive care after exposure. After Colorado legalized recreational cannabis in 2012, unintended health consequences included increased healthcare visits due to ingestion, cyclic vomiting syndrome due to frequent use of high THC concentration products, and burns from THC extraction-related explosions. Colorado also reported increased healthcare visits due to accidental ingestion of edible products by children and increased unintentional overdoses, while among adults, concentrated products such as resins and liquid concentrates resulted in increased toxic exposures. In 2013, there had been a 16 percent increase in hospitalizations in Colorado due to cannabis in pediatric patients and a 30 percent annual increase in call rates to poison control. COVID-19 shelter-in-place orders may also have affected cannabis exposures; with increased cannabis use anticipated in response to lock downs. Mental health stress associated with the COVID-19 pandemic has been associated with increased desire to use recreational substances, especially in states where cannabis is legal.